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09-02-2011, 06:47 PM
I have not ventured into the zooplankton/phytoplankton production, but there are a number of folks that have....maybe we can get them to post.
Making refractometer calibration fluid is easy - if you have the right tools. An analytical grade balance to precisely weigh out the NaCl and a volumetric flask is needed.
However, if you want to give it a try, you can get pretty close by dissolving 3.65 grams of NaCl into 96.35 mL of RODI. If you don't have the tools for this type of measurement, you can do the following*:
1. Measure 1/4 cup of Morton's Iodized Salt (about 73.1 g)
2. Add 1 teaspoon of salt (making about 79.3 g total salt)
3. Measure the full volume of a plastic 2-L Coke or Diet Coke bottle filled with purified freshwater (about 2104.4 g)
4. Dissolve the total salt (79.3 g) in the total water volume (2104 g) to make an approximately 3.65 weight percent solution of NaCl. The volume of this solution will be slightly larger than the Coke bottle, so dissolve it in another container.
*from Reef Aquarium Salinity: Homemade Calibration Standards
Since most people don't have the necessary instruments and tools to precisely weigh chemicals to make calibration solutions, they buy them to ensure quality and precision. I will have some available at the Ann Arbor swap if you would like to get it there.
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Last edited by BeakerBob; 09-02-2011 at 10:32 PM.