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Breeding Journal: Nannochloropsis


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  1. #1
    dputt88 - Reefkeeper CR Member
    Join Date
    Aug 2012
    Location
    Romeo MI
    Posts
    873
    First Name
    Doug
    Awards Tank of the Month - January 2013 Tank of the Month Post and Reply Award - Finding Nemo PAR contest Fishbowl Drawing Winner - Summer Swap Fishbowl Winner (Red Dragon)

    Default Breeding Journal: Nannochloropsis

    <!-- BEGIN TEMPLATE: formoutput -->
    Breeding Journal DataSheet

    This first post should be updated regularly to include new information as events take place
    or changes are made to your system


    General
    Species: Nannochloropsis
    Social Structure: phytoplankton
    Size of Individuals: 2-6 microns
    Age of Individuals: 30 days
    Date added to Tank: every other day

    Broodstock Tank Details
    Size of Tank: 5 liters
    Substrate Details: none
    Filtration Details: none
    Water Changes: none
    Water Temperature: room temp
    Lighting: exo terra CFL and 18" t-8 plant bulb
    Lighting Cycle: 24/7 occasional respiration period
    Other Tank Inhabitants: none

    Broodstock Feeding Details
    Food Types: miracle grow
    Feeding Schedule: once at start of culture

    Spawning Details
    Date of First Spawn: Dec 2011
    Spawn Time of Day: when i have the time
    Dates of Consecutive Spawns: every other week
    Courtship Details: none
    Egg Size: none
    Egg Color: none
    Egg Count: none

    Hatch Details
    Hatch Date: none
    Hatch Time of Day: none
    # Days after Spawn: none
    Larvae Description: none


    Larval Tank Details
    Temperature: room temp
    Size of Larval Tank: none
    Substrate Details: none
    Other Tank Decor: none
    Filtration Details: none
    Lighting: same
    Lighting Cycle: same
    Water Changes: none

    Larval Feeding Details
    Food Types: miracle grow
    Feeding Schedule: same

    Metamorphosis/Settlement
    Date of Settlement Start: none
    Days after Hatch: none
    Date of Settlement End: none
    Description of Fry: green

    Grow-Out Tank Details
    Temperature: same
    Size of Grow-Out Tank: same
    Substrate Details: none
    Other Tank Decor: none
    Filtration Details: none
    Lighting: same
    Lighting Cycle: same
    Water Changes: none
    Size at Transfer: none
    Age at Transfer: none

    Grow-Out Feeding Details
    Food Types: same
    Feeding Schedule: same

    Additional Information
    Miscellaneous Information: well since december i have been culturing phytoplankton. i lost one culture due to contamination in febuary 2012, but since then i have had one continual culture and its been going strong. so heres how i do it.

    i bought a Nanno disc from Florida Aqua Farms.

    1331350981 - Breeding Journal: Nannochloropsis

    1331479558 - Breeding Journal: Nannochloropsis

    then i let it soak in fresh water overnight, scraped half of the disc and added it to a 200 ml starter culture, then added it to a one liter culture, and now i use 5L cultures. my culture media is old tank water that has been diluted down to a SG of <1.019 and sterilized by boiling for 10 min. after it cools i add 5 ml miracle grow per 1L media. i put it on a stir plate with a sterile stir bar and aerate the media using sterile ridged tubing. to serilize misc materials i use 70% ethanol ( no, 100% is not better, nor is methanol).

    so how about some more pics.

    2012 03 16082134 - Breeding Journal: Nannochloropsis

    2012 03 19191608 - Breeding Journal: Nannochloropsis

    1332619632 - Breeding Journal: Nannochloropsis

    current setup


    2012 06 17104122 - Breeding Journal: Nannochloropsis

    2012 07 11200408 - Breeding Journal: Nannochloropsis


    so thats how its been going for the past 6 months, i make lots of phytoplankton. after harsesting i save a small portion of the culture and add it to fresh media and let it grow. typically it takes about 10 days to get to max density. the portion that is then harvested is allow to settle, all of the excess media is then poured out and i add a small amount of nutrient deficient media to help extend its shelf life and prevent nutrients from making their way into the tank.

    any questions just ask.
    <!-- END TEMPLATE: formoutput -->
    Last edited by dputt88; 09-04-2012 at 12:51 PM.

  2. #2
    dputt88 - Reefkeeper CR Member
    Join Date
    Aug 2012
    Location
    Romeo MI
    Posts
    873
    First Name
    Doug
    Awards Tank of the Month - January 2013 Tank of the Month Post and Reply Award - Finding Nemo PAR contest Fishbowl Drawing Winner - Summer Swap Fishbowl Winner (Red Dragon)

    Default

    edited due to photos being out of order.

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